mkn45_5k_500k_rpc - MKN-45, 5k cells, 500k read-pairs-per-cell

Alerts

The analysis detected 1 warning.
AlertValueDetail
Number of cells5219The recovered cell count is ideally between 250 and 5000. A high value indicates an overloading of cells, or severe underloading, or a problem during library preparation.
5,219
Estimated Number of Cells
2.20
Median estimated CNV resolution (in Mb)
1.88
Median ploidy

Sequencing

Total reads
Total number of sequencing reads
Read length
The median number of alignable bases in read 1 (or read 2)
Fraction of Q30 bases
The fraction of total bases with quality score at least 30 for read 1 (or read 2)
Reads with valid barcodes
The number of reads that could be confidently associated with a valid barcode
Total reads3,004,809,734
R1 length (in bp)84
R2 length (in bp)100
Fraction of Q30 R1 bases91.3%
Fraction of Q30 R2 bases89.3%
Reads with valid barcodes2,603,491,258 (86.6%)

Cells and Mapping

Cells detected
The number of barcodes associated with partitions containing cells
Reads from cells
The number of reads with a valid barcode that are associated with cell-containing partitions
Total mapped de-duplicated reads in cells
Total number of reads that have mapping quality at least 30 and are not duplicates that are associated with cell-containing partitions
Median duplicate fraction per cell
Median over cells of the fraction of duplicate reads
Median effective reads per Mb
Median over cells of the number of reads per cell with mapping quality at least 30 and not duplicate divided by the genome size in megabases
Median average ploidy
Median of the average ploidy per cell distribution
Median estimated CNV resolution per cell
The approximate size of copy number events (in Mb) that can be profiled accurately in single cells based on the sequencing depth and library quality, as determined by a theoretical model
Fraction of noisy cells
Fraction of cells in the dataset that have high values of DIMAPD or where the copy number estimation procedure may be unreliable
Fraction of genome mappable
The fraction of 20kb bins of the genome that are at least 70% mappable
Cells detected5219
Reads from cells2,498,735,000 (83.2%)
Total mapped de-duplicated reads in cells2,155,934,261 (71.7%)
Mean mapped de-duplicated reads per cell413,093
Median duplicate fraction per cell4.5%
Median unmapped fraction per cell1.0%
Median effective reads per Mb121
Median average ploidy1.88
Median estimated CNV resolution (in Mb)2.20
Fraction of noisy cells14.6%
Fraction of genome mappable89.7%

Cell Plots

110010k110100100010k100k1M
NoiseCellsBarcode RankBarcodesMapped Reads
24605001000150020002500
Cell Ploidy HistogramAverage PloidyCells
0.511.50200400600800
DIMAPD (noisy)DIMAPDCell DIMAPD HistogramDIMAPDCells

Sample Details

Sample IDmkn45_5k_500k_rpc
DescriptionMKN-45, 5k cells, 500k read-pairs-per-cell
FASTQ path
‎/mnt/scratch2/cellranger-dna/fastqs/mkn45_5k‎
Reference path
‎/mnt/scratch2/cellranger-dna/references/refdata-GRCh37-1.0.0‎
Cell Ranger DNA version1.1.0
OrganismHomo_sapiens
AssemblyGRCh37
Annotationensembl.org/pub/release-87