Cell Ranger · hgmm_6k · 1:1 mixture of fresh frozen human (HEK293T) and mouse (NIH3T3) cells
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SUMMARY      ANALYSIS
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Estimated Number of Cells

6,806

Mean Reads per Cell

54,870
Number of Reads
Total number of single-end reads that were sequenced.
Valid Barcodes
Fraction of reads with barcodes that match the whitelist.
hg19 Reads Mapped Confidently to Transcriptome
Fraction of reads that mapped to a unique gene in the hg19 transcriptome with a high mapping quality score as reported by the aligner.
mm10 Reads Mapped Confidently to Transcriptome
Fraction of reads that mapped to a unique gene in the mm10 transcriptome with a high mapping quality score as reported by the aligner.
hg19 Reads Mapped Confidently to Exonic Regions
Fraction of reads that mapped to the exonic regions of the genome with a high mapping quality score as reported by the aligner.
mm10 Reads Mapped Confidently to Exonic Regions
Fraction of reads that mapped to the exonic regions of the genome with a high mapping quality score as reported by the aligner.
hg19 Reads Mapped Confidently to Intronic Regions
Fraction of reads that mapped to the intronic regions of the hg19 genome with a high mapping quality score as reported by the aligner.
mm10 Reads Mapped Confidently to Intronic Regions
Fraction of reads that mapped to the intronic regions of the mm10 genome with a high mapping quality score as reported by the aligner.
hg19 Reads Mapped Confidently to Intergenic Regions
Fraction of reads that mapped to the intergenic regions of the hg19 genome with a high mapping quality score as reported by the aligner.
mm10 Reads Mapped Confidently to Intergenic Regions
Fraction of reads that mapped to the intergenic regions of the mm10 genome with a high mapping quality score as reported by the aligner.
Sequencing Saturation
The fraction of reads originating from an already-observed UMI. This is a function of library complexity and sequencing depth. More specifically, this is the fraction of confidently mapped, valid cell-barcode, valid UMI reads that had a non-unique (cell-barcode, UMI, gene). This metric was called "cDNA PCR Duplication" in versions of Cell Ranger prior to 1.2.
Q30 Bases in Barcode
Fraction of bases with Q-score at least 30 in the cell barcode sequences.
Q30 Bases in RNA Read
Fraction of bases with Q-score at least 30 in the RNA read sequences. This is Illumina R1 for the Single Cell 3' v1 chemistry and Illumina R2 for the Single Cell 3' v2 chemistry.
Q30 Bases in Sample Index
Fraction of bases with Q-score at least 30 in the sample index sequences.
Q30 Bases in UMI
Fraction of bases with Q-score at least 30 in the UMI sequences.

Sequencing

Number of Reads 373,449,584
Valid Barcodes 98.3%
hg19 Reads Mapped Confidently to Transcriptome 42.5%
mm10 Reads Mapped Confidently to Transcriptome 22.5%
hg19 Reads Mapped Confidently to Exonic Regions 45.5%
mm10 Reads Mapped Confidently to Exonic Regions 23.7%
hg19 Reads Mapped Confidently to Intronic Regions 6.9%
mm10 Reads Mapped Confidently to Intronic Regions 3.3%
hg19 Reads Mapped Confidently to Intergenic Regions 1.7%
mm10 Reads Mapped Confidently to Intergenic Regions 1.1%
Sequencing Saturation 24.1%
Q30 Bases in Barcode 97.9%
Q30 Bases in RNA Read 81.2%
Q30 Bases in Sample Index 96.7%
Q30 Bases in UMI 98.0%
GEMs with >0 Cells
The number of barcodes associated with at least one cell.
GEMs with >1 Cell
The estimated number of barcodes that are associated with multiple cells.
Fraction GEMs with >1 Cell
The fraction of cell-associated barcodes estimated to be associated with more than one cell.
Mean UMI Count Purity
Among single-cell GEM barcodes, the mean fraction of UMI counts coming from the transcriptome of the cell inferred to be in the GEM, as opposed to a transcriptome that should not be present in the cell.

GEM Partitions

GEMs with >0 Cells 6,806
GEMs with >1 Cell 345
Fraction GEMs with >1 Cell 5.1%
Mean UMI Count Purity 98.2%
 
Estimated Number of Cells
The total number of barcodes associated with cell-containing partitions, estimated from the barcode count distribution.
hg19 Estimated Number of Cell Partitions
The number of barcodes associated with hg19 cell-containing partitions, estimated from the barcode count distribution.
mm10 Estimated Number of Cell Partitions
The number of barcodes associated with mm10 cell-containing partitions, estimated from the barcode count distribution.
hg19 Fraction Reads in Cells
The fraction of barcoded, confidently mapped reads with hg19 cell-associated barcodes.
mm10 Fraction Reads in Cells
The fraction of barcoded, confidently mapped reads with mm10 cell-associated barcodes.
Mean Reads per Cell
The total number of sequenced reads divided by the number of barcodes associated with cell-containing partitions.
hg19 Median Genes per Cell
The median number of genes detected per hg19 cell-associated barcode. Detection is defined as the presence of at least 1 UMI count.
mm10 Median Genes per Cell
The median number of genes detected per mm10 cell-associated barcode. Detection is defined as the presence of at least 1 UMI count.
hg19 Total Genes Detected
The number of hg19 genes with at least one count in any cell.
mm10 Total Genes Detected
The number of mm10 genes with at least one count in any cell.
hg19 Median UMI Counts per Cell
The median number of UMI counts per hg19 cell-associated barcode.
mm10 Median UMI Counts per Cell
The median number of UMI counts per mm10 cell-associated barcode.

Cells

Estimated Number of Cells 6,806
hg19 Estimated Number of Cell Partitions 3,572
mm10 Estimated Number of Cell Partitions 3,408
hg19 Fraction Reads in Cells 86.9%
mm10 Fraction Reads in Cells 88.8%
Mean Reads per Cell 54,870
hg19 Median Genes per Cell 5,009
mm10 Median Genes per Cell 3,475
hg19 Total Genes Detected 20,781
mm10 Total Genes Detected 16,274
hg19 Median UMI Counts per Cell 27,949
mm10 Median UMI Counts per Cell 15,883

Sample

Name hgmm_6k
Description 1:1 mixture of fresh frozen human (HEK293T) and mouse (NIH3T3) cells
Transcriptome hg19, mm10
Chemistry Single Cell 3' v2
Cell Ranger Version 1.2.0

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