Estimated Number of Cells

1,011

Mean Reads per Cell

83,592

Median Genes per Cell

2,058

Sequencing

Number of Reads 84,512,390
Valid Barcodes 97.0%
Sequencing Saturation 66.0%
Q30 Bases in Barcode 97.4%
Q30 Bases in RNA Read 95.3%
Q30 Bases in Sample Index 92.1%
Q30 Bases in UMI 97.3%

Mapping

Reads Mapped to Genome 95.3%
Reads Mapped Confidently to Genome 89.5%
Reads Mapped Confidently to Intergenic Regions 5.0%
Reads Mapped Confidently to Intronic Regions 22.7%
Reads Mapped Confidently to Exonic Regions 61.8%
Reads Mapped Confidently to Transcriptome 59.2%
Reads Mapped Antisense to Gene 1.1%
 

Cells

100% Cells(739/739)
CellsBackground
Estimated Number of Cells 1,011
Fraction Reads in Cells 89.4%
Mean Reads per Cell 83,592
Median Genes per Cell 2,058
Total Genes Detected 19,436
Median UMI Counts per Cell 7,610

Sample

Name heart_1k_v3
Description Cells from the whole heart of an E18 mouse
Transcriptome mm10
Chemistry Single Cell 3' v3
Cell Ranger Version 3.0.0

Clustering Type:

Shown here are the total UMI counts for each cell-barcode. Cells with greater UMI counts likely have higher RNA content than cells with fewer UMI counts. The axes correspond to the 2-dimensional embedding produced by the t-SNE algorithm. In this space, pairs of cells that are close to each other have more similar gene expression profiles than cells that are distant from each other. The display is limited to a random subset of 10000 cells.

t-SNE Projection of Cells Colored by UMI Counts

UMI counts: 1,661
10k20k30k40k
 
These are the assignments of each cell-barcode a clusters by an automated clustering algorithm. The clustering groups together cells that have similar expression profiles. The axes correspond to the 2-dimensional embedding produced by the t-SNE algorithm. In this space, pairs of cells that are close to each other have more similar gene expression profiles than cells that are distant from each other. The display is limited to a random subset of 10000 cells and K-means up to K=20. Please use Loupe(tm) Cell Browser to view the entire dataset.

t-SNE projection of Cells Colored by Automated Clustering

1 - 232 cells 2 - 172 cells 3 - 155 cells 4 - 138 cells 5 - 131 cells 6 - 102 cells 7 - 81 cells
The differential expression analysis seeks to find, for each cluster, genes that are more highly expressed in that cluster relative to the rest of the sample. Here a differential expression test was performed between each cluster and the rest of the sample for each gene. The Log2 fold-change (L2FC) is an estimate of the log2 ratio of expression in a cluster to that in all other cells. A value of 1.0 indicates 2-fold greater expression in the cluster of interest. The p-value is a measure of the statistical significance of the expression difference and is based on a negative binomial test. The p-value reported here has been adjusted for multiple testing via the Benjamini-Hochberg procedure. In this table you can click on a column to sort by that value. Also, in this table genes were filtered by (Mean UMI counts > 1.0) and the top N genes by L2FC for each cluster were retained. Genes with L2FC < 0 or adjusted p-value >= 0.10 were grayed out. The number of top genes shown per cluster, N, is set to limit the number of table entries shown to 10000; N=10000/K^2 where K is the number of clusters. N can range from 1 to 50. For the full table, please refer to the "differential_expression.csv" files produced by the pipeline.

Top Genes By Cluster (Log2 fold-change, p-value)

    Cluster 1Cluster 2Cluster 3Cluster 4Cluster 5Cluster 6Cluster 7  

Gene ID

Gene name

L2FC

p-value

L2FC

p-value

L2FC

p-value

L2FC

p-value

L2FC

p-value

L2FC

p-value

L2FC

p-value

ENSMUSG00000104876Trdc 3.163e-58-3.036e-34-6.032e-16-0.141e+00-2.095e-01-0.401e+00-5.283e-17
ENSMUSG00000040204Pclaf 3.152e-68-2.533e-31-8.847e-28-4.094e-10-1.806e-010.258e-01-2.806e-11
ENSMUSG00000076749Tcrg-C1 3.006e-47-2.662e-24-6.301e-13-0.211e+00-1.369e-01-0.471e+00-4.551e-12
ENSMUSG00000027203Dut 2.974e-67-2.363e-30-8.735e-29-1.585e-03-2.702e-01-1.212e-01-1.575e-05
ENSMUSG00000055612Cdca7 2.847e-56-2.771e-33-5.044e-16-0.783e-01-0.911e+000.445e-01-3.111e-11
ENSMUSG00000001228Uhrf1 2.841e-58-2.295e-27-7.708e-23-1.274e-02-1.995e-01-0.181e+00-2.312e-08
ENSMUSG00000005410Mcm5 2.829e-57-1.926e-20-7.173e-20-3.861e-08-2.463e-01-0.071e+00-2.353e-08
ENSMUSG00000076752Tcrg-C2 2.746e-41-2.456e-22-6.301e-130.366e-01-4.182e-01-0.549e-01-4.551e-12
ENSMUSG00000020649Rrm2 2.582e-44-3.052e-37-3.354e-11-3.803e-08-1.309e-010.891e-01-0.692e-01
ENSMUSG00000041859Mcm3 2.525e-47-1.642e-15-6.273e-19-2.681e-05-2.563e-01-0.291e+00-1.791e-05
ENSMUSG00000032397Tipin 2.514e-48-1.438e-13-5.671e-18-3.057e-07-4.146e-02-0.924e-01-1.892e-06
ENSMUSG00000023132Gzma 2.495e-22-1.791e-08-6.887e-130.031e+00-2.648e-01-0.969e-01-4.812e-10
ENSMUSG00000029730Mcm7 2.491e-48-2.162e-25-7.673e-23-1.411e-02-1.457e-010.494e-01-1.614e-05
ENSMUSG00000025747Tyms 2.482e-44-1.713e-16-4.831e-16-3.332e-07-1.051e+000.485e-01-2.706e-10
ENSMUSG00000029687Ezh2 2.476e-47-2.224e-26-7.752e-23-0.496e-01-1.239e-010.061e+00-1.741e-05
ENSMUSG00000032011Thy1 2.452e-48-3.233e-49-8.514e-280.989e-03-0.651e+000.248e-01-6.773e-30
ENSMUSG00000024338Psmb8 2.446e-49-2.556e-35-6.523e-250.922e-02-1.228e-01-0.854e-01-4.773e-23
ENSMUSG00000022881Rfc4 2.434e-39-1.736e-15-6.381e-15-1.708e-03-2.683e-01-1.401e-01-0.818e-02
ENSMUSG00000067367Lyar 2.373e-42-1.414e-12-6.033e-18-1.941e-03-2.912e-01-1.162e-01-1.512e-04
ENSMUSG00000015749Anp32e 2.364e-46-2.355e-31-5.857e-250.051e+00-1.616e-01-0.131e+00-1.432e-04
ENSMUSG00000094777Hist1h2ap 2.364e-39-2.781e-33-6.342e-20-0.694e-01-1.349e-011.634e-04-3.004e-11
ENSMUSG00000026355Mcm6 2.341e-42-1.653e-16-6.507e-21-1.996e-04-1.915e-01-0.081e+00-1.329e-04
ENSMUSG00000074476Spc24 2.331e-41-1.821e-18-7.222e-20-1.616e-03-1.288e-01-0.021e+00-1.106e-03
ENSMUSG00000030707Coro1a 2.323e-46-2.837e-43-8.463e-331.084e-03-0.681e+00-0.081e+00-5.321e-28
ENSMUSG00000028333Anp32b 2.324e-45-1.463e-14-4.187e-19-1.145e-02-2.383e-01-1.585e-02-1.706e-06
ENSMUSG00000056394Lig1 2.264e-38-1.302e-10-6.313e-19-3.645e-08-1.865e-010.179e-01-2.105e-07
ENSMUSG00000028832Stmn1 2.262e-41-1.492e-14-7.161e-31-1.021e-01-2.792e-01-1.202e-01-1.309e-04
ENSMUSG00000045826Ptprcap 2.242e-39-2.601e-33-6.964e-231.283e-04-1.081e+00-0.746e-01-5.212e-22
ENSMUSG00000037894H2afz 2.241e-42-1.377e-13-6.471e-31-1.273e-02-2.473e-01-1.242e-01-1.497e-05
ENSMUSG00000018189Uchl5 2.243e-35-1.601e-13-4.953e-14-0.753e-01-2.423e-01-0.855e-01-1.186e-03
ENSMUSG00000027342Pcna 2.231e-38-1.793e-18-6.614e-21-1.743e-03-1.398e-010.652e-01-1.396e-04
ENSMUSG00000017716Birc5 2.231e-36-1.532e-13-6.511e-21-0.882e-01-1.806e-01-0.776e-01-1.416e-04
ENSMUSG00000025364Pa2g4 2.232e-42-1.412e-13-8.185e-27-1.312e-02-2.473e-01-1.812e-02-1.057e-03
ENSMUSG00000005481Ddx39 2.232e-39-1.755e-18-7.322e-21-0.654e-01-1.735e-01-0.231e+00-1.232e-03
ENSMUSG00000023505Cdca3 2.228e-36-1.942e-19-5.221e-16-1.314e-02-1.776e-010.141e+00-0.662e-01
ENSMUSG00000005696Sh2d1a 2.202e-30-3.881e-44-5.524e-151.677e-06-0.581e+00-0.151e+00-6.367e-19
ENSMUSG00000025574Tk1 2.209e-34-1.334e-10-6.493e-16-3.675e-07-2.783e-01-0.321e+00-0.973e-02
ENSMUSG00000029322Plac8 2.194e-24-1.433e-08-6.934e-16-0.381e+000.995e-01-2.653e-02-4.856e-14
ENSMUSG00000002870Mcm2 2.187e-34-1.404e-11-5.591e-15-2.088e-04-2.154e-01-0.331e+00-1.081e-02
ENSMUSG00000042489Clspn 2.179e-30-2.371e-22-5.022e-13-2.641e-040.191e+001.752e-04-2.137e-06
ENSMUSG00000030978Rrm1 2.156e-37-1.541e-14-7.455e-22-2.696e-06-1.626e-010.317e-01-0.972e-02
ENSMUSG00000033220Rac2 2.152e-38-2.619e-36-8.592e-281.543e-06-1.298e-01-1.508e-02-5.388e-26
ENSMUSG00000054717Hmgb2 2.142e-39-1.709e-19-5.672e-28-0.545e-01-1.139e-010.475e-01-2.197e-09
ENSMUSG00000053398Phgdh 2.135e-37-0.896e-06-5.281e-19-1.644e-03-3.748e-02-2.198e-03-2.812e-11
ENSMUSG00000006715Gmnn 2.131e-34-2.091e-22-4.106e-14-2.075e-04-0.731e+000.928e-02-0.768e-02
ENSMUSG00000033685Ucp2 2.122e-38-2.005e-24-2.569e-100.661e-01-1.218e-01-0.421e+00-3.783e-18
ENSMUSG00000038482Tfdp1 2.128e-33-1.153e-08-6.682e-17-1.462e-02-1.388e-01-0.865e-01-1.812e-05
ENSMUSG00000000782Tcf7 2.127e-38-3.857e-65-7.703e-301.665e-07-0.191e+000.121e+00-5.532e-28
ENSMUSG00000031403Dkc1 2.113e-32-1.413e-11-5.591e-15-1.265e-02-1.477e-01-0.291e+00-1.243e-03
ENSMUSG00000069272Hist1h2ae 2.097e-29-3.109e-36-6.041e-17-0.556e-01-0.531e+002.205e-07-3.782e-13
ENSMUSG00000019773Fbxo5 1.822e-18-2.633e-22-5.611e-12-2.019e-03-0.011e+002.533e-08-2.591e-06
ENSMUSG00000049539Hist1h1a 1.741e-14-3.893e-30-5.862e-10-0.411e+001.453e-012.561e-07-3.528e-08
ENSMUSG00000001403Ube2c 1.561e-17-1.549e-13-1.196e-03-0.141e+00-1.419e-010.121e+00-0.721e-01
ENSMUSG00000068855Hist2h2ac 1.552e-10-2.271e-12-5.031e-07-1.718e-020.839e-012.595e-07-2.081e-03
ENSMUSG00000022945Chaf1b 1.553e-03-1.071e-01-5.827e-03-1.211e+001.895e-01-0.058e-01-1.874e-01
ENSMUSG00000074071Fam169b 1.502e-11-3.075e-23-5.822e-100.396e-011.056e-012.551e-07-4.077e-09
ENSMUSG00000042029Ncapg2 1.333e-11-1.661e-11-6.175e-13-2.862e-040.061e+002.654e-10-1.744e-04
ENSMUSG00000022372Sla 1.312e-11-3.771e-36-6.018e-132.413e-130.688e-010.872e-01-4.672e-12
ENSMUSG00000104413Gm37065 1.313e-10-3.448e-29-5.628e-112.652e-15-0.161e+000.091e+00-5.883e-12
ENSMUSG00000022148Fyb 1.282e-13-3.082e-38-6.704e-201.681e-061.727e-021.587e-04-5.362e-20
ENSMUSG00000022468Endou 1.232e-11-3.883e-47-5.527e-162.621e-170.441e+000.574e-01-5.367e-18
ENSMUSG00000022014Epsti1 1.223e-09-2.242e-17-5.802e-112.519e-14-0.491e+00-0.598e-01-4.466e-11
ENSMUSG00000024670Cd6 1.216e-07-3.392e-22-3.936e-072.442e-090.976e-010.793e-01-5.778e-10
ENSMUSG00000037572Wdhd1 1.164e-07-1.922e-11-5.248e-09-0.706e-010.937e-012.541e-07-0.961e-01
ENSMUSG00000046591Ticrr 1.166e-06-2.371e-12-4.894e-07-1.797e-020.987e-013.231e-11-2.543e-04
ENSMUSG00000030775Trat1 1.142e-08-2.747e-24-6.003e-122.472e-130.758e-010.555e-01-5.259e-13
ENSMUSG00000015314Slamf6 1.142e-08-3.984e-39-6.431e-131.579e-051.711e-012.391e-07-5.092e-13
ENSMUSG00000037474Dtl 1.056e-08-1.721e-13-5.816e-16-0.705e-011.164e-012.699e-11-2.061e-05
ENSMUSG00000053044Cd8b1 1.051e-09-5.657e-86-6.804e-232.902e-240.816e-010.722e-01-6.316e-26
ENSMUSG00000020297Nsg2 1.021e-07-3.559e-38-5.471e-132.694e-180.787e-010.891e-01-4.726e-14
ENSMUSG00000030745Il21r 0.978e-05-2.761e-16-5.039e-082.667e-120.441e+000.972e-01-5.291e-08
ENSMUSG00000001741Il16 0.893e-05-2.912e-23-5.631e-102.541e-131.075e-011.352e-02-4.886e-11
ENSMUSG00000015476Prrt1 0.863e-04-2.732e-17-5.123e-082.471e-101.463e-011.266e-02-3.783e-07
ENSMUSG00000032503Arpp21 0.823e-06-3.922e-54-7.852e-222.132e-111.784e-022.209e-08-3.394e-13
ENSMUSG00000018168Ikzf3 0.798e-04-3.531e-24-5.219e-092.474e-111.135e-012.115e-05-5.478e-10
ENSMUSG00000061311Rag1 0.783e-05-3.049e-35-5.151e-152.862e-211.183e-010.663e-01-5.411e-18
ENSMUSG00000022283Pabpc1 0.772e-06-0.184e-01-1.812e-06-0.291e+00-0.671e+000.111e+00-1.036e-03
ENSMUSG00000048251Bcl11b 0.762e-05-3.213e-41-6.431e-201.782e-072.076e-032.431e-09-4.266e-17
ENSMUSG00000026012Cd28 0.752e-04-3.272e-32-6.552e-142.151e-091.876e-022.191e-06-5.212e-14
ENSMUSG00000093674Rpl41 0.753e-06-0.088e-01-1.215e-04-0.357e-01-1.765e-01-2.193e-03-0.631e-01
ENSMUSG00000052949Rnf157 0.722e-03-2.343e-16-6.213e-111.442e-031.254e-012.775e-09-3.652e-08
ENSMUSG00000028150Rorc 0.703e-03-2.925e-20-5.454e-092.424e-101.373e-011.811e-03-2.525e-05
ENSMUSG00000034438Gbp8 0.704e-03-2.254e-14-4.533e-082.299e-091.891e-011.472e-02-4.792e-09
ENSMUSG00000072553Gm525 0.693e-03-3.512e-29-6.562e-123.345e-23-0.731e+000.259e-01-5.233e-12
ENSMUSG00000047139Cd24a 0.682e-04-1.696e-160.979e-040.981e-020.121e+000.415e-01-1.379e-04
ENSMUSG00000039356Exosc2 0.666e-02-1.472e-04-5.744e-06-0.819e-010.221e+002.652e-040.209e-01
ENSMUSG00000079110Capn3 0.639e-03-2.797e-19-5.336e-093.148e-200.748e-01-0.021e+00-2.994e-06
ENSMUSG00000037318Traf3ip3 0.619e-03-3.027e-22-5.343e-093.119e-210.629e-010.922e-01-4.592e-09
ENSMUSG00000038489Polr2l 0.582e-01-0.831e-01-4.851e-03-0.861e+002.492e-011.472e-01-0.431e+00
ENSMUSG00000056895Hist3h2ba 0.575e-03-2.132e-19-5.922e-152.569e-160.181e+001.397e-03-1.644e-04
ENSMUSG00000019845Tube1 0.561e-02-3.176e-25-4.643e-092.104e-081.642e-012.646e-09-3.564e-08
ENSMUSG00000030579Tyrobp 0.562e-01-0.257e-01-5.632e-041.341e-011.984e-01-2.776e-01-3.071e-02
ENSMUSG00000035242Oaz1 0.559e-04-0.629e-04-0.219e-010.237e-01-1.268e-01-1.722e-020.334e-01
ENSMUSG00000038352Arl5c 0.557e-03-2.622e-26-5.834e-153.116e-260.727e-010.594e-01-4.764e-15
ENSMUSG00000091831Gm4707 0.528e-02-1.281e-04-4.457e-060.121e+000.511e+003.202e-10-2.094e-03
ENSMUSG00000052331Ankrd44 0.494e-02-0.412e-01-5.792e-101.015e-021.702e-011.303e-02-4.036e-09
ENSMUSG00000085715Tsix 0.483e-02-1.135e-06-4.111e-090.821e-012.043e-022.353e-07-2.892e-07
ENSMUSG00000035697Arhgap45 0.463e-02-2.322e-22-6.783e-162.292e-121.864e-022.157e-07-5.039e-16
ENSMUSG00000035891Cerk 0.454e-02-1.346e-08-5.693e-112.421e-120.649e-010.703e-01-1.367e-03
ENSMUSG00000033952Aspm 0.459e-02-0.594e-02-4.024e-07-0.391e+000.591e+002.542e-07-1.136e-02
ENSMUSG00000034634Ly6d 0.431e-01-1.264e-05-5.194e-093.012e-13-0.391e+00-1.913e-01-4.713e-09
ENSMUSG00000030798Cd37 0.439e-02-2.115e-13-4.463e-082.755e-141.433e-011.353e-02-4.132e-08
ENSMUSG00000001524Gtf2h4 0.436e-02-1.922e-14-6.303e-132.641e-151.632e-011.001e-01-2.443e-06
ENSMUSG00000019505Ubb 0.421e-020.194e-01-2.019e-080.287e-01-1.188e-01-0.131e+00-1.121e-03
ENSMUSG00000098120Gm5914 0.401e-01-1.698e-10-4.561e-082.714e-141.204e-010.922e-01-2.806e-06
ENSMUSG00000025357Dgka 0.382e-01-1.543e-08-5.522e-092.432e-101.861e-011.333e-02-4.181e-08
ENSMUSG00000029007Agtrap 0.351e-01-0.932e-04-5.511e-102.454e-13-0.571e+000.466e-01-1.406e-03
ENSMUSG00000072620Slfn2 0.342e-01-1.501e-08-5.778e-112.784e-161.045e-010.872e-01-4.023e-09
ENSMUSG00000016756Cmah 0.322e-01-2.873e-19-4.201e-073.034e-181.453e-011.683e-03-5.451e-09
ENSMUSG00000050592Fam78a 0.284e-01-2.762e-16-3.912e-062.862e-141.364e-011.482e-02-1.021e-01
ENSMUSG00000034317Trim59 0.234e-01-1.029e-05-2.765e-061.073e-021.046e-012.611e-08-1.467e-03
ENSMUSG00000033545Znrf1 0.233e-01-1.313e-09-6.111e-162.423e-141.542e-011.405e-03-2.594e-08
ENSMUSG00000047246Hist1h2be 0.236e-01-1.405e-04-4.627e-05-0.061e+002.803e-023.072e-06-2.547e-03
ENSMUSG00000024399Ltb 0.224e-01-2.051e-16-6.611e-142.863e-191.492e-011.631e-03-5.281e-14
ENSMUSG00000048546Tob2 0.224e-01-0.651e-02-6.626e-140.553e-011.552e-012.606e-09-2.271e-05
ENSMUSG00000033760Rbm4b 0.224e-01-0.516e-02-5.862e-110.357e-011.849e-022.327e-07-1.681e-03
ENSMUSG00000027347Rasgrp1 0.225e-01-3.228e-25-5.831e-102.511e-112.453e-032.436e-07-5.089e-11
ENSMUSG00000041264Uspl1 0.225e-01-1.251e-05-5.103e-081.482e-031.403e-012.721e-08-2.165e-04
ENSMUSG00000058427Cxcl2 0.169e-011.031e-01-5.727e-02-5.236e-011.736e-01-4.451e+00-2.794e-01
ENSMUSG00000097206Gm26699 0.157e-01-1.093e-05-5.711e-101.073e-022.162e-022.731e-09-2.637e-06
ENSMUSG00000054892Txk 0.146e-01-2.522e-21-6.215e-132.669e-161.886e-022.381e-07-5.461e-13
ENSMUSG00000063856Gpx1 0.136e-01-0.117e-010.845e-03-0.321e+00-1.517e-01-0.441e+00-0.622e-01
ENSMUSG00000034833Tespa1 0.137e-01-2.844e-24-5.977e-122.706e-161.984e-022.428e-08-4.211e-10
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ENSMUSG00000007877Tcap -7.214e-37-2.125e-08-2.682e-03-3.521e-02-1.891e+00-2.742e-016.357e-68
ENSMUSG00000094686Ccl21a -7.232e-088.413e-15-5.621e+00-5.131e+00-2.501e+00-4.351e+00-5.878e-01
ENSMUSG00000022803Popdc2 -7.298e-43-3.168e-16-5.091e-06-3.604e-03-1.971e+00-3.824e-027.491e-98
ENSMUSG00000052957Gas1 -7.314e-586.651e-88-5.115e-08-6.209e-06-4.585e-01-4.431e-02-2.614e-04
ENSMUSG00000048126Col6a3 -7.323e-588.924e-108-6.122e-09-5.631e-05-4.014e-01-4.855e-03-6.381e-10
ENSMUSG00000006221Hspb7 -7.353e-64-2.592e-15-6.741e-10-6.252e-06-2.628e-01-5.472e-037.007e-116
ENSMUSG00000021388Aspn -7.377e-378.232e-65-5.767e-06-5.273e-03-2.641e+00-4.499e-02-5.011e-05
ENSMUSG00000063011Msln -7.409e-328.511e-56-6.524e-05-4.444e-02-4.411e+00-5.252e-01-6.781e-05
ENSMUSG00000023046Igfbp6 -7.413e-449.012e-81-6.543e-07-6.054e-04-4.428e-01-5.273e-02-6.794e-08
ENSMUSG00000036446Lum -7.431e-347.794e-59-4.829e-05-5.334e-03-3.701e+00-4.551e-01-4.073e-04
ENSMUSG00000059741Myl3 -7.462e-83-6.012e-49-3.678e-07-6.773e-08-1.781e+00-4.993e-038.733e-172
ENSMUSG00000091898Tnnc1 -7.471e-96-3.967e-35-4.453e-10-5.102e-07-1.931e+00-3.846e-037.825e-174
ENSMUSG00000020096Tbata -7.483e-347.862e-56-5.025e-04-6.111e-02-5.481e+00-4.754e-01-5.272e-04
ENSMUSG00000021186Fbln5 -7.482e-357.802e-59-6.619e-06-4.113e-02-2.481e+00-4.332e-01-6.862e-06
ENSMUSG00000073940Hbb-bt -7.494e-137-5.181e-637.554e-196-8.841e-181.344e-01-6.182e-06-10.175e-38
ENSMUSG00000060572Mfap2 -7.504e-728.226e-127-6.896e-12-4.402e-05-3.774e-01-5.628e-04-6.151e-12
ENSMUSG00000062694Cav3 -7.515e-27-2.413e-07-2.719e-03-3.823e-02-1.191e+00-3.042e-016.723e-54
ENSMUSG00000036256Igfbp7 -7.553e-788.046e-135-6.165e-12-5.674e-07-2.716e-01-4.303e-03-7.414e-15
ENSMUSG00000061462Obscn -7.577e-50-3.382e-19-3.795e-06-3.888e-04-1.251e+00-3.105e-027.443e-111
ENSMUSG00000029019Nppb -7.629e-24-6.875e-16-4.002e-02-4.511e-01-2.891e+00-3.748e-019.531e-55
ENSMUSG00000041616Nppa -7.662e-29-6.451e-17-3.973e-02-5.659e-02-4.761e+00-5.198e-019.311e-61
ENSMUSG00000035458Tnni3 -7.668e-98-3.609e-31-4.073e-09-6.641e-09-2.208e-01-5.861e-047.681e-170
ENSMUSG00000030399Ckm -7.672e-92-4.241e-36-2.965e-06-5.746e-08-1.521e+00-5.703e-047.811e-166
ENSMUSG00000032334Loxl1 -7.698e-667.692e-113-6.082e-10-5.583e-06-1.958e-01-3.808e-03-4.741e-09
ENSMUSG00000038583Pln -7.701e-74-3.175e-21-5.356e-10-7.189e-08-1.741e+00-4.081e-027.389e-130
ENSMUSG00000071317Bves -7.713e-36-3.092e-13-4.512e-05-4.024e-03-0.801e+00-3.248e-027.432e-86
ENSMUSG00000053093Myh7 -7.734e-67-7.253e-46-2.601e-03-7.211e-060.191e+00-4.852e-027.923e-117
ENSMUSG00000067276Capn6 -7.764e-437.342e-71-5.561e-06-4.073e-03-3.458e-01-4.294e-02-3.222e-04
ENSMUSG00000042045Sln -7.771e-34-3.053e-09-3.101e-02-6.085e-03-3.451e+00-4.303e-017.252e-57
ENSMUSG00000017969Ptgis -7.811e-678.661e-121-6.201e-10-5.702e-06-3.085e-01-4.932e-03-5.455e-11
ENSMUSG00000028116Myoz2 -7.859e-47-5.521e-27-4.071e-05-5.173e-04-1.951e+00-3.397e-029.045e-115
ENSMUSG00000016349Eef1a2 -7.926e-41-3.675e-18-3.724e-05-4.232e-03-0.591e+00-1.863e-017.564e-98
ENSMUSG00000041476Smpx -8.053e-68-4.833e-34-5.856e-10-5.368e-06-2.738e-01-4.586e-038.872e-155
ENSMUSG00000020911Krt19 -8.064e-487.054e-71-5.867e-07-6.372e-04-4.748e-01-5.593e-02-2.773e-03
ENSMUSG00000001119Col6a1 -8.155e-737.837e-120-5.951e-10-6.464e-07-3.834e-01-4.684e-03-4.032e-08
ENSMUSG00000021268Meg3 -8.281e-917.658e-135-5.341e-10-6.763e-08-3.676e-01-5.986e-04-4.333e-09
ENSMUSG00000019787Trdn -8.289e-53-2.938e-18-2.748e-05-4.591e-04-1.371e+00-3.811e-027.121e-118
ENSMUSG00000040666Sh3bgr -8.292e-53-3.493e-19-4.094e-06-5.608e-05-2.978e-01-4.821e-027.826e-110
ENSMUSG00000068614Actc1 -8.306e-118-3.188e-26-4.793e-11-6.221e-09-3.116e-01-5.184e-047.465e-165
ENSMUSG00000024803Ankrd1 -8.334e-59-5.574e-31-2.958e-04-6.225e-05-5.606e-01-4.445e-028.801e-119
ENSMUSG00000026414Tnnt2 -8.362e-116-3.373e-28-3.254e-07-6.474e-10-2.209e-01-5.532e-047.428e-165
ENSMUSG00000013936Myl2 -8.381e-91-6.263e-50-3.896e-07-6.035e-07-2.969e-01-5.552e-039.237e-179
ENSMUSG00000051747Ttn -8.384e-68-2.602e-11-2.239e-03-7.502e-06-0.301e+00-6.735e-036.502e-76
ENSMUSG00000040752Myh6 -8.423e-84-2.415e-13-2.341e-03-8.317e-09-2.511e+00-6.536e-046.575e-101
ENSMUSG00000042985Upk3b -8.429e-489.608e-85-6.812e-07-5.311e-03-4.698e-01-5.543e-02-7.062e-08
ENSMUSG00000026043Col3a1 -8.721e-1079.023e-176-6.033e-13-6.545e-09-5.722e-01-6.994e-05-7.519e-19
ENSMUSG00000020469Myl7 -8.727e-65-2.235e-08-4.641e-05-7.282e-05-6.667e-01-6.182e-026.671e-69
ENSMUSG00000001506Col1a1 -8.763e-889.439e-149-7.447e-13-7.535e-08-5.105e-01-6.769e-04-8.283e-16
ENSMUSG00000023224Serping1 -8.775e-549.377e-97-5.574e-08-5.088e-05-2.458e-01-4.301e-02-5.836e-09
ENSMUSG00000030470Csrp3 -8.784e-88-3.882e-30-4.444e-09-5.832e-07-2.468e-01-5.051e-038.012e-160
ENSMUSG00000071984Fndc1 -8.825e-4710.424e-85-5.635e-07-5.134e-04-3.517e-01-4.353e-02-5.881e-07
ENSMUSG00000018893Mb -8.841e-58-6.359e-35-4.227e-06-6.742e-05-1.641e+00-4.962e-029.306e-127
ENSMUSG00000009281Rarres2 -8.932e-629.796e-109-7.329e-10-6.839e-06-5.205e-01-6.056e-03-5.992e-09
ENSMUSG00000022225Cma1 -9.178e-0410.771e-07-5.971e+00-5.481e+00-3.861e+00-4.701e+00-6.231e+00
ENSMUSG00000030116Mfap5 -9.203e-359.794e-61-6.004e-05-4.502e-02-3.881e+00-4.732e-01-6.251e-05
ENSMUSG00000029661Col1a2 -9.201e-1029.107e-164-6.386e-13-6.891e-08-4.415e-01-5.857e-04-7.965e-18
ENSMUSG00000042436Mfap4 -9.416e-468.686e-76-6.229e-07-5.727e-04-4.109e-01-3.362e-01-4.885e-06
ENSMUSG00000031375Bgn -9.805e-888.939e-144-7.602e-13-6.112e-07-2.488e-01-5.339e-04-7.861e-15
ENSMUSG00000061816Myl1 -9.936e-43-3.558e-13-4.412e-04-6.242e-03-4.611e+00-5.469e-027.973e-74
ENSMUSG00000019929Dcn -10.723e-10410.206e-170-6.526e-13-7.613e-09-5.993e-01-6.842e-04-7.774e-17
This plot shows the Sequencing Saturation metric as a function of downsampled sequencing depth in mean reads per cell, up to the observed sequencing depth. Sequencing Saturation is a measure of the observed library complexity, and approaches 1.0 (100%) when all converted mRNA transcripts have been sequenced. The slope of the curve near the endpoint can be interpreted as an upper bound to the benefit to be gained from increasing the sequencing depth beyond this point. The dotted line is drawn at a value reasonably approximating the saturation point.

Sequencing Saturation

020k40k60k80k00.20.40.60.81
Mean Reads per CellSequencing Saturation
 
This plot shows the Median Genes per Cell as a function of downsampled sequencing depth in mean reads per cell, up to the observed sequencing depth. The slope of the curve near the endpoint can be interpreted as an upper bound to the benefit to be gained from increasing the sequencing depth beyond this point.

Median Genes per Cell

020k40k60k80k0500100015002000
Mean Reads per CellMedian Genes per Cellmm10