Estimated Number of Cells

1,063

Mean Reads per Cell

74,047

Sequencing

Number of Reads 78,712,670
Valid Barcodes 98.0%
Sequencing Saturation 26.0%
Q30 Bases in Barcode 96.8%
Q30 Bases in RNA Read 95.5%
Q30 Bases in Sample Index 93.5%
Q30 Bases in UMI 96.8%

Mapping

Reads Mapped to Genome 97.3%
hg19 Reads Mapped to Genome 59.5%
mm10 Reads Mapped to Genome 38.1%
Reads Mapped Confidently to Genome 87.6%
hg19 Reads Mapped Confidently to Genome 55.5%
mm10 Reads Mapped Confidently to Genome 32.1%
Reads Mapped Confidently to Intergenic Regions 2.9%
hg19 Reads Mapped Confidently to Intergenic Regions 1.8%
mm10 Reads Mapped Confidently to Intergenic Regions 1.1%
Reads Mapped Confidently to Intronic Regions 21.0%
hg19 Reads Mapped Confidently to Intronic Regions 14.0%
mm10 Reads Mapped Confidently to Intronic Regions 7.1%
Reads Mapped Confidently to Exonic Regions 63.7%
hg19 Reads Mapped Confidently to Exonic Regions 39.7%
mm10 Reads Mapped Confidently to Exonic Regions 24.0%
Reads Mapped Confidently to Transcriptome 60.2%
hg19 Reads Mapped Confidently to Transcriptome 37.5%
mm10 Reads Mapped Confidently to Transcriptome 22.7%
Reads Mapped Antisense to Gene 1.1%

GEM Partitions

GEMs with >0 Cells 1,063
GEMs with >1 Cell 4
Fraction GEMs with >1 Cell 0.4%
Fraction GEMs with >1 Cell (Lower Bound) 0.0%
Fraction GEMs with >1 Cell (Upper Bound) 1.0%
Mean UMI Count Purity 99.6%
 

Cells

100% Cells(929/929)
CellsBackground
Estimated Number of Cells 1,063
hg19 Estimated Number of Cell Partitions 469
mm10 Estimated Number of Cell Partitions 596
Fraction Reads in Cells 88.2%
hg19 Fraction Reads in Cells 85.5%
mm10 Fraction Reads in Cells 90.7%
Mean Reads per Cell 74,047
hg19 Median Genes per Cell 6,765
mm10 Median Genes per Cell 4,742
hg19 Total Genes Detected 22,553
mm10 Total Genes Detected 19,146
hg19 Median UMI Counts per Cell 40,990
mm10 Median UMI Counts per Cell 20,170

Sample

Name 1k_hgmm_v3
Description 1:1 mixture of fresh frozen human (HEK293T) and mouse (NIH3T3) cells
Transcriptome hg19_and_mm10
Chemistry Single Cell 3' v3
Cell Ranger Version 3.0.2

Clustering Type:

Each point represents a cell-barcode. The axes measure the total UMI counts in each cell-barcode that mapped to each transcriptome reference. The points are colored by the number of inferred cells in the GEM associated with each barcode. A multiplet represents either a GEM inferred to have encapsulated >1 cell or a barcode sequence that was shared by multiple single-cell GEMs.

Cell UMI Counts

050k100k150k020k40k60k80k
hg19 UMI countsmm10 UMI countshg19mm10Multiplet
 
Shown here are the total UMI counts for each cell-barcode. Cells with greater UMI counts likely have higher RNA content than cells with fewer UMI counts. The axes correspond to the 2-dimensional embedding produced by the t-SNE algorithm. In this space, pairs of cells that are close to each other have more similar gene expression profiles than cells that are distant from each other. The display is limited to a random subset of 10000 cells.

t-SNE Projection of Cells Colored by UMI Counts

UMI counts: 1,107
10k20k30k40k50k60k70k
 
These are the assignments of each cell-barcode a clusters by an automated clustering algorithm. The clustering groups together cells that have similar expression profiles. The axes correspond to the 2-dimensional embedding produced by the t-SNE algorithm. In this space, pairs of cells that are close to each other have more similar gene expression profiles than cells that are distant from each other. The display is limited to a random subset of 10000 cells and K-means up to K=20. Please use Loupe(tm) Cell Browser to view the entire dataset.

t-SNE projection of Cells Colored by Automated Clustering

1 - 309 cells 2 - 215 cells 3 - 207 cells 4 - 193 cells 5 - 139 cells
The differential expression analysis seeks to find, for each cluster, genes that are more highly expressed in that cluster relative to the rest of the sample. Here a differential expression test was performed between each cluster and the rest of the sample for each gene. The Log2 fold-change (L2FC) is an estimate of the log2 ratio of expression in a cluster to that in all other cells. A value of 1.0 indicates 2-fold greater expression in the cluster of interest. The p-value is a measure of the statistical significance of the expression difference and is based on a negative binomial test. The p-value reported here has been adjusted for multiple testing via the Benjamini-Hochberg procedure. In this table you can click on a column to sort by that value. Also, in this table genes were filtered by (Mean UMI counts > 1.0) and the top N genes by L2FC for each cluster were retained. Genes with L2FC < 0 or adjusted p-value >= 0.10 were grayed out. The number of top genes shown per cluster, N, is set to limit the number of table entries shown to 10000; N=10000/K^2 where K is the number of clusters. N can range from 1 to 50. For the full table, please refer to the "differential_expression.csv" files produced by the pipeline.

Top Genes By Cluster (Log2 fold-change, p-value)

    Cluster 1Cluster 2Cluster 3Cluster 4Cluster 5  

Gene ID

Gene name

L2FC

p-value

L2FC

p-value

L2FC

p-value

L2FC

p-value

L2FC

p-value

hg19_ENSG00000197061hg19_HIST1H4C 5.781e-129-9.401e-86-5.266e-39-8.686e-30-2.252e-03
hg19_ENSG00000136897hg19_MRPL50 5.509e-101-6.542e-44-5.376e-31-5.562e-15-1.963e-03
hg19_ENSG00000103187hg19_COTL1 5.482e-83-8.101e-40-7.679e-31-6.543e-14-1.341e-01
hg19_ENSG00000101843hg19_PSMD10 5.445e-102-6.192e-44-6.762e-35-5.637e-16-1.661e-02
hg19_ENSG00000092010hg19_PSME1 5.352e-96-7.041e-44-6.034e-32-6.483e-16-1.433e-02
hg19_ENSG00000135900hg19_MRPL44 5.305e-86-7.711e-40-4.941e-25-6.143e-14-1.533e-02
hg19_ENSG00000196636hg19_ACN9 5.283e-95-6.456e-43-5.617e-31-5.486e-15-1.592e-02
hg19_ENSG00000243927hg19_MRPS6 5.262e-110-7.583e-54-5.152e-34-7.021e-19-1.522e-02
hg19_ENSG00000103005hg19_USB1 5.253e-88-6.811e-40-4.795e-26-6.258e-15-1.642e-02
hg19_ENSG00000086189hg19_DIMT1 5.232e-77-7.644e-37-6.201e-26-5.079e-12-1.271e-01
hg19_ENSG00000079459hg19_FDFT1 5.222e-99-6.218e-45-5.783e-33-6.653e-17-1.463e-02
hg19_ENSG00000184281hg19_TSSC4 5.223e-94-7.042e-44-6.025e-32-6.484e-16-1.286e-02
hg19_ENSG00000134333hg19_LDHA 5.205e-140-7.023e-81-5.792e-52-6.524e-28-1.502e-02
hg19_ENSG00000158710hg19_TAGLN2 5.183e-120-8.475e-70-6.294e-46-6.176e-22-1.296e-02
hg19_ENSG00000108518hg19_PFN1 5.183e-147-7.753e-95-5.414e-52-7.596e-34-1.462e-02
hg19_ENSG00000143256hg19_PFDN2 5.169e-132-7.323e-73-6.305e-51-5.592e-22-1.383e-02
hg19_ENSG00000110104hg19_CCDC86 5.162e-88-8.102e-44-6.674e-32-4.953e-13-1.211e-01
hg19_ENSG00000205352hg19_PRR13 5.151e-100-7.483e-50-6.053e-35-5.926e-17-1.257e-02
hg19_ENSG00000154719hg19_MRPL39 5.155e-98-6.853e-47-6.412e-35-6.879e-18-1.209e-02
hg19_ENSG00000203760hg19_CENPW 5.157e-130-6.546e-67-5.993e-48-7.571e-26-1.393e-02
hg19_ENSG00000065978hg19_YBX1 5.144e-147-7.582e-97-5.579e-55-7.303e-34-1.393e-02
hg19_ENSG00000175334hg19_BANF1 5.144e-133-7.467e-76-6.332e-52-7.322e-27-1.256e-02
hg19_ENSG00000143179hg19_UCK2 5.136e-101-6.745e-48-5.301e-32-6.775e-18-1.374e-02
hg19_ENSG00000197958hg19_RPL12 5.131e-151-7.893e-106-5.583e-56-6.953e-34-1.383e-02
hg19_ENSG00000205302hg19_SNX2 5.138e-113-8.762e-59-4.749e-34-6.201e-19-1.492e-02
hg19_ENSG00000175581hg19_MRPL48 5.124e-92-6.488e-43-5.631e-30-5.507e-15-1.375e-02
hg19_ENSG00000214194hg19_LINC00998 5.111e-68-6.994e-34-7.565e-27-6.432e-12-1.043e-01
hg19_ENSG00000169714hg19_CNBP 5.114e-126-7.354e-69-5.337e-43-5.377e-21-1.502e-02
hg19_ENSG00000118939hg19_UCHL3 5.103e-124-8.001e-68-5.572e-43-6.445e-23-1.294e-02
hg19_ENSG00000125821hg19_DTD1 5.102e-106-7.551e-53-5.309e-35-6.992e-19-1.275e-02
hg19_ENSG00000101444hg19_AHCY 5.092e-125-7.387e-68-5.954e-46-6.149e-23-1.275e-02
hg19_ENSG00000187514hg19_PTMA 5.097e-149-7.501e-101-5.892e-59-7.389e-36-1.283e-02
hg19_ENSG00000112306hg19_RPS12 5.093e-149-7.494e-102-5.031e-49-6.847e-34-1.502e-02
hg19_ENSG00000174444hg19_RPL4 5.087e-146-7.701e-98-5.731e-56-7.092e-33-1.294e-02
hg19_ENSG00000140350hg19_ANP32A 5.085e-119-7.444e-67-7.011e-49-7.479e-25-1.101e-01
hg19_ENSG00000105700hg19_KXD1 5.063e-107-7.972e-57-6.221e-39-5.097e-17-1.218e-02
hg19_ENSG00000116649hg19_SRM 5.065e-125-7.408e-75-6.427e-52-6.291e-24-1.209e-02
hg19_ENSG00000232119hg19_MCTS1 5.061e-113-7.092e-61-5.242e-38-5.794e-20-1.423e-02
hg19_ENSG00000179958hg19_DCTPP1 5.062e-121-7.937e-72-5.822e-45-6.372e-23-1.228e-02
hg19_ENSG00000147224hg19_PRPS1 5.061e-105-6.883e-53-5.861e-37-6.325e-19-1.228e-02
hg19_ENSG00000107833hg19_NPM3 5.053e-110-7.355e-57-6.182e-40-5.796e-19-1.178e-02
hg19_ENSG00000185088hg19_RPS27L 5.052e-132-6.956e-76-7.264e-59-6.305e-26-1.169e-02
hg19_ENSG00000115541hg19_HSPE1 5.055e-132-7.561e-84-5.691e-50-6.916e-29-1.266e-02
hg19_ENSG00000187837hg19_HIST1H1C 5.041e-86-6.492e-42-5.742e-30-5.934e-15-1.271e-01
hg19_ENSG00000172354hg19_GNB2 5.044e-123-7.746e-69-6.993e-50-5.866e-22-1.101e-01
hg19_ENSG00000069482hg19_GAL 5.041e-104-8.955e-58-6.935e-41-6.394e-19-0.992e-01
hg19_ENSG00000105887hg19_MTPN 5.033e-101-9.416e-60-5.804e-37-7.844e-21-1.082e-01
hg19_ENSG00000175063hg19_UBE2C 5.032e-104-6.735e-58-6.082e-41-6.449e-21-1.251e-01
hg19_ENSG00000101150hg19_TPD52L2 5.022e-66-7.592e-33-6.158e-24-6.021e-11-0.964e-01
hg19_ENSG00000118680hg19_MYL12B 5.026e-116-7.492e-63-5.253e-39-5.939e-21-1.325e-02
hg19_ENSG00000204764hg19_RANBP17 2.033e-12-6.663e-18-6.238e-14-5.102e-062.107e-05
hg19_ENSG00000160298hg19_C21orf58 2.036e-16-6.812e-27-6.372e-20-6.256e-102.096e-06
hg19_ENSG00000189283hg19_FHIT 1.971e-11-5.867e-18-4.842e-12-5.301e-062.117e-05
hg19_ENSG00000184351hg19_KRTAP19-1 1.962e-06-6.865e-12-4.842e-07-3.716e-032.096e-03
hg19_ENSG00000188206hg19_HNRNPU-AS1 1.954e-12-5.912e-20-4.252e-12-5.948e-082.087e-05
hg19_ENSG00000225210hg19_AL589743.1 1.941e-15-5.203e-22-5.192e-17-4.645e-082.102e-06
hg19_ENSG00000229807hg19_XIST 1.932e-17-7.924e-61-4.631e-24-7.441e-202.122e-06
hg19_ENSG00000105576hg19_TNPO2 1.928e-11-6.725e-18-4.289e-11-5.163e-062.156e-05
hg19_ENSG00000182871hg19_COL18A1 1.926e-15-7.399e-27-5.379e-18-4.823e-082.171e-06
hg19_ENSG00000009413hg19_REV3L 1.914e-12-6.832e-20-3.572e-10-5.275e-072.103e-05
hg19_ENSG00000111215hg19_PRR4 1.894e-15-5.972e-23-6.548e-19-5.411e-082.232e-07
hg19_ENSG00000105429hg19_MEGF8 1.875e-17-6.887e-27-5.447e-19-5.313e-092.255e-09
hg19_ENSG00000203930hg19_LINC00632 1.865e-11-7.311e-21-4.294e-12-4.751e-062.192e-05
hg19_ENSG00000198563hg19_DDX39B 1.866e-14-7.523e-27-4.072e-14-5.952e-092.191e-06
hg19_ENSG00000221978hg19_CCNL2 1.862e-14-6.428e-26-5.403e-18-4.862e-082.233e-07
hg19_ENSG00000168255hg19_POLR2J3 1.855e-21-8.462e-54-5.563e-33-6.902e-182.257e-10
hg19_ENSG00000266967hg19_AARSD1 1.832e-08-6.757e-16-4.735e-10-5.192e-052.261e-04
hg19_ENSG00000223705hg19_NSUN5P1 1.838e-14-4.072e-16-4.381e-13-4.852e-072.155e-07
hg19_ENSG00000131584hg19_ACAP3 1.811e-09-6.481e-16-5.041e-11-4.919e-062.301e-05
hg19_ENSG00000177302hg19_TOP3A 1.782e-10-6.443e-21-4.001e-11-5.881e-072.251e-05
hg19_ENSG00000144395hg19_CCDC150 1.781e-06-6.215e-12-4.183e-07-4.654e-042.305e-04
hg19_ENSG00000158805hg19_ZNF276 1.742e-07-5.426e-13-4.409e-09-4.868e-052.321e-04
hg19_ENSG00000128191hg19_DGCR8 1.732e-08-8.166e-23-4.391e-11-6.609e-082.343e-05
hg19_ENSG00000215154hg19_AC141586.5 1.724e-10-6.732e-19-6.297e-15-5.179e-072.416e-07
hg19_ENSG00000228253hg19_MT-ATP8 1.711e-16-5.981e-31-6.141e-24-5.017e-112.385e-11
hg19_ENSG00000109065hg19_NAT9 1.684e-09-5.898e-18-4.872e-12-3.745e-052.374e-06
hg19_ENSG00000253203hg19_GUSBP3 1.672e-09-6.762e-19-5.332e-13-5.201e-062.456e-07
hg19_ENSG00000198886hg19_MT-ND4 1.642e-21-8.732e-117-5.924e-57-5.372e-242.455e-14
hg19_ENSG00000270069hg19_RP6-99M1.2 1.633e-13-7.185e-34-4.742e-20-6.622e-122.451e-09
hg19_ENSG00000168679hg19_SLC16A4 1.611e-10-6.214e-19-4.774e-13-4.651e-062.502e-09
hg19_ENSG00000161996hg19_WDR90 1.592e-05-5.053e-10-4.621e-07-4.497e-042.491e-04
hg19_ENSG00000168096hg19_ANKS3 1.598e-06-6.319e-13-5.881e-09-4.753e-042.554e-05
hg19_ENSG00000198763hg19_MT-ND2 1.572e-19-8.359e-101-5.596e-51-5.304e-232.521e-14
hg19_ENSG00000198786hg19_MT-ND5 1.534e-18-8.476e-99-6.163e-55-5.389e-232.576e-15
hg19_ENSG00000174501hg19_ANKRD36C 1.444e-06-6.714e-16-6.282e-12-3.564e-042.661e-06
hg19_ENSG00000198727hg19_MT-CYB 1.448e-17-9.017e-119-5.818e-55-5.321e-232.651e-16
hg19_ENSG00000212907hg19_MT-ND4L 1.441e-15-7.991e-63-5.302e-37-4.965e-172.643e-15
hg19_ENSG00000211459hg19_MT-RNR1 1.385e-15-9.021e-112-5.295e-47-5.508e-242.714e-17
hg19_ENSG00000198840hg19_MT-ND3 1.342e-14-8.763e-108-6.042e-55-5.091e-212.758e-18
hg19_ENSG00000210082hg19_MT-RNR2 1.332e-14-9.111e-119-5.571e-52-5.342e-232.763e-18
hg19_ENSG00000198804hg19_MT-CO1 1.316e-14-9.237e-119-6.272e-58-5.098e-222.782e-18
hg19_ENSG00000198712hg19_MT-CO2 1.292e-13-8.722e-113-6.095e-58-4.954e-222.801e-18
hg19_ENSG00000198938hg19_MT-CO3 1.292e-13-8.918e-116-6.361e-60-4.935e-222.811e-18
hg19_ENSG00000146830hg19_GIGYF1 1.272e-04-4.281e-09-3.854e-07-4.324e-042.742e-06
hg19_ENSG00000248527hg19_MTATP6P1 1.277e-13-8.855e-97-6.171e-53-4.861e-192.839e-19
hg19_ENSG00000198888hg19_MT-ND1 1.268e-13-9.087e-112-5.702e-51-5.015e-212.836e-19
hg19_ENSG00000198899hg19_MT-ATP6 1.223e-12-8.602e-110-6.202e-57-5.003e-212.871e-19
hg19_ENSG00000104899hg19_AMH 1.152e-04-4.613e-12-4.175e-09-4.644e-052.892e-08
hg19_ENSG00000270641hg19_TSIX 0.783e-03-5.017e-18-4.262e-12-3.453e-053.246e-14
hg19_ENSG00000198695hg19_MT-ND6 0.767e-05-8.792e-70-6.596e-45-4.462e-143.341e-23
mm10_ENSMUSG00000098178mm10_Gm42418 -3.819e-921.253e-101.432e-111.983e-13-0.745e-01
mm10_ENSMUSG00000023764mm10_Sfi1 -6.067e-851.335e-091.925e-162.013e-11-3.395e-04
mm10_ENSMUSG00000034488mm10_Edil3 -6.074e-811.722e-141.501e-092.052e-11-4.241e-04
mm10_ENSMUSG00000036800mm10_Fam135b -6.181e-751.054e-052.952e-330.612e-01-4.352e-04
mm10_ENSMUSG00000019817mm10_Plagl1 -6.364e-1180.731e-032.662e-361.725e-09-5.443e-07
mm10_ENSMUSG00000064357mm10_mt-Atp6 -6.381e-1291.441e-111.852e-161.963e-11-4.606e-07
mm10_ENSMUSG00000019518mm10_Ap4m1 -6.452e-891.613e-131.653e-122.031e-11-4.425e-05
mm10_ENSMUSG00000022032mm10_Scara5 -6.462e-800.206e-012.974e-371.876e-09-4.212e-04
mm10_ENSMUSG00000006800mm10_Sulf2 -6.496e-1270.893e-052.361e-291.998e-13-5.568e-08
mm10_ENSMUSG00000046743mm10_Fat4 -6.555e-861.021e-052.692e-321.272e-04-3.298e-04
mm10_ENSMUSG00000020034mm10_Tcp11l2 -6.553e-790.745e-032.492e-241.991e-09-4.302e-04
mm10_ENSMUSG00000029641mm10_Rasl11a -6.563e-1390.822e-042.813e-411.368e-06-6.244e-09
mm10_ENSMUSG00000038388mm10_Mpp6 -6.607e-793.203e-460.881e-030.218e-01-3.082e-03
mm10_ENSMUSG00000032449mm10_Slc25a36 -6.625e-891.674e-141.654e-121.978e-11-4.366e-05
mm10_ENSMUSG00000047793mm10_Sned1 -6.629e-1700.454e-022.756e-431.901e-11-6.246e-11
mm10_ENSMUSG00000097296mm10_Gm26532 -6.643e-541.031e-031.851e-082.497e-10-4.125e-03
mm10_ENSMUSG00000027868mm10_Tbx15 -6.655e-951.338e-092.022e-171.937e-10-4.812e-05
mm10_ENSMUSG00000040945mm10_Rcc2 -6.651e-902.879e-420.888e-041.004e-03-3.404e-04
mm10_ENSMUSG00000013846mm10_St3gal1 -6.673e-1110.916e-052.668e-351.528e-07-5.151e-06
mm10_ENSMUSG00000037894mm10_H2afz -6.685e-1613.309e-660.514e-020.665e-02-7.173e-11
mm10_ENSMUSG00000032238mm10_Rora -6.692e-920.968e-052.655e-291.442e-05-3.852e-04
mm10_ENSMUSG00000041120mm10_Nbl1 -6.714e-1290.682e-032.902e-441.404e-06-4.686e-07
mm10_ENSMUSG00000002020mm10_Ltbp2 -6.739e-650.932e-032.621e-201.541e-04-2.622e-02
mm10_ENSMUSG00000064339mm10_mt-Rnr2 -6.753e-2411.314e-111.832e-182.169e-16-4.018e-09
mm10_ENSMUSG00000026576mm10_Atp1b1 -6.785e-1530.792e-042.761e-421.513e-07-6.476e-10
mm10_ENSMUSG00000002732mm10_Fkbp7 -6.785e-1271.044e-072.651e-391.345e-06-5.272e-07
mm10_ENSMUSG00000035373mm10_Ccl7 -6.793e-932.895e-310.818e-031.077e-03-5.868e-06
mm10_ENSMUSG00000024754mm10_Tmem2 -6.832e-981.383e-101.906e-172.042e-12-4.582e-05
mm10_ENSMUSG00000022330mm10_Osr2 -6.844e-870.967e-052.619e-301.562e-06-4.321e-04
mm10_ENSMUSG00000015568mm10_Lpl -6.844e-1440.749e-042.823e-411.481e-06-6.333e-09
mm10_ENSMUSG00000067367mm10_Lyar -6.852e-923.062e-421.021e-040.286e-01-3.812e-04
mm10_ENSMUSG00000029177mm10_Cenpa -6.852e-335.303e-34-1.319e-02-1.175e-01-4.011e-01
mm10_ENSMUSG00000021281mm10_Tnfaip2 -6.856e-950.837e-042.788e-331.432e-05-3.822e-04
mm10_ENSMUSG00000037278mm10_Tmem97 -6.895e-803.112e-400.769e-030.728e-02-4.372e-04
mm10_ENSMUSG00000028565mm10_Nfia -6.891e-1120.552e-022.932e-431.562e-07-5.052e-06
mm10_ENSMUSG00000036446mm10_Lum -6.908e-1821.071e-072.626e-391.344e-06-6.382e-11
mm10_ENSMUSG00000021806mm10_Nid2 -6.912e-1231.064e-072.202e-252.022e-13-4.243e-06
mm10_ENSMUSG00000028370mm10_Pappa -6.913e-1290.841e-042.631e-341.673e-08-5.778e-08
mm10_ENSMUSG00000017002mm10_Slpi -6.934e-570.861e-023.047e-200.889e-02-4.893e-03
mm10_ENSMUSG00000022687mm10_Boc -6.955e-1040.871e-042.704e-361.545e-07-4.698e-06
mm10_ENSMUSG00000019942mm10_Cdk1 -6.958e-583.811e-390.177e-010.121e+00-4.115e-03
mm10_ENSMUSG00000020642mm10_Rnf144a -6.965e-821.023e-052.722e-311.292e-04-4.122e-04
mm10_ENSMUSG00000025223mm10_Ldb1 -6.983e-1161.184e-082.009e-202.161e-14-5.131e-06
mm10_ENSMUSG00000029657mm10_Hsph1 -6.994e-833.242e-480.623e-020.699e-02-4.152e-04
mm10_ENSMUSG00000100009mm10_Gm7967 -7.006e-1150.871e-042.434e-291.942e-11-5.161e-06
mm10_ENSMUSG00000055897mm10_Ppp4r1l-ps -7.011e-1130.978e-062.314e-271.992e-12-3.972e-05
mm10_ENSMUSG00000032855mm10_Pkd1 -7.021e-1301.513e-141.834e-181.974e-13-5.341e-07
mm10_ENSMUSG00000053113mm10_Socs3 -7.052e-1910.791e-042.782e-451.492e-07-6.494e-12
mm10_ENSMUSG00000037465mm10_Klf10 -7.056e-1011.396e-101.836e-152.136e-13-4.791e-05
mm10_ENSMUSG00000023067mm10_Cdkn1a -7.062e-761.831e-121.401e-062.084e-09-4.544e-04
mm10_ENSMUSG00000035385mm10_Ccl2 -7.073e-714.349e-44-0.732e-010.178e-01-5.489e-04
mm10_ENSMUSG00000056758mm10_Hmga2 -7.083e-1093.419e-560.333e-010.735e-02-4.412e-05
mm10_ENSMUSG00000097206mm10_Gm26699 -7.105e-821.452e-091.816e-132.105e-11-4.252e-04
mm10_ENSMUSG00000024587mm10_Nars -7.102e-1072.907e-460.893e-040.954e-03-3.844e-05
mm10_ENSMUSG00000026069mm10_Il1rl1 -7.103e-974.205e-66-0.394e-010.031e+00-4.132e-04
mm10_ENSMUSG00000062248mm10_Cks2 -7.148e-644.452e-60-0.414e-01-0.418e-01-3.882e-03
mm10_ENSMUSG00000097324mm10_Carmn -7.185e-1041.352e-091.896e-162.112e-12-4.928e-06
mm10_ENSMUSG00000024620mm10_Pdgfrb -7.201e-1281.481e-121.803e-162.054e-13-5.522e-07
mm10_ENSMUSG00000019961mm10_Tmpo -7.223e-1143.171e-560.902e-040.344e-01-4.962e-06
mm10_ENSMUSG00000005481mm10_Ddx39 -7.241e-1053.032e-460.905e-040.696e-02-4.986e-06
mm10_ENSMUSG00000001403mm10_Ube2c -7.256e-225.961e-23-2.702e-02-1.031e+00-2.991e+00
mm10_ENSMUSG00000025784mm10_Clec3b -7.265e-156-0.254e-013.344e-611.759e-10-4.746e-08
mm10_ENSMUSG00000062901mm10_Klhl24 -7.273e-1291.212e-092.098e-242.002e-13-3.641e-05
mm10_ENSMUSG00000001056mm10_Nhp2 -7.274e-1182.922e-400.953e-040.823e-02-5.885e-07
mm10_ENSMUSG00000025068mm10_Gsto1 -7.342e-1293.003e-520.827e-040.887e-03-5.502e-07
mm10_ENSMUSG00000003032mm10_Klf4 -7.363e-1771.034e-072.718e-431.271e-05-6.833e-11
mm10_ENSMUSG00000028716mm10_Pdzk1ip1 -7.365e-1090.664e-033.047e-461.259e-05-4.846e-06
mm10_ENSMUSG00000025289mm10_Prdx4 -7.375e-2601.081e-082.221e-311.987e-15-6.643e-16
mm10_ENSMUSG00000036570mm10_Fxyd1 -7.371e-1220.693e-032.865e-411.535e-07-5.346e-07
mm10_ENSMUSG00000031451mm10_Gas6 -7.384e-1370.903e-052.748e-401.432e-06-5.705e-08
mm10_ENSMUSG00000000031mm10_H19 -7.382e-1160.736e-032.771e-281.589e-06-3.861e-04
mm10_ENSMUSG00000022037mm10_Clu -7.399e-820.707e-033.087e-391.142e-03-4.133e-04
mm10_ENSMUSG00000029484mm10_Anxa3 -7.412e-612.983e-260.871e-020.906e-02-4.153e-03
mm10_ENSMUSG00000017493mm10_Igfbp4 -7.421e-840.892e-032.662e-221.584e-05-5.169e-05
mm10_ENSMUSG00000071708mm10_Sms -7.431e-1671.132e-082.223e-271.932e-12-4.911e-08
mm10_ENSMUSG00000064356mm10_mt-Atp8 -7.433e-1251.698e-141.651e-111.949e-10-4.052e-05
mm10_ENSMUSG00000034614mm10_Pik3ip1 -7.466e-871.003e-052.282e-222.044e-11-4.201e-04
mm10_ENSMUSG00000026043mm10_Col3a1 -7.467e-2751.284e-122.074e-271.944e-14-6.891e-17
mm10_ENSMUSG00000018819mm10_Lsp1 -7.481e-1021.134e-072.125e-212.096e-13-4.642e-05
mm10_ENSMUSG00000023832mm10_Acat2 -7.531e-1452.914e-521.036e-060.713e-02-5.851e-08
mm10_ENSMUSG00000027797mm10_Dclk1 -7.585e-1182.952e-430.887e-040.891e-02-5.549e-07
mm10_ENSMUSG00000056481mm10_Cd248 -7.592e-2270.822e-052.692e-471.601e-09-5.112e-11
mm10_ENSMUSG00000019929mm10_Dcn -7.614e-3110.912e-062.624e-451.621e-09-6.852e-19
mm10_ENSMUSG00000078190mm10_Dnm3os -7.616e-1701.342e-111.997e-221.992e-13-5.473e-09
mm10_ENSMUSG00000026456mm10_Cyb5r1 -7.632e-943.152e-490.672e-020.862e-02-4.376e-05
mm10_ENSMUSG00000042436mm10_Mfap4 -7.646e-1250.485e-022.641e-322.101e-12-5.604e-07
mm10_ENSMUSG00000027750mm10_Postn -7.674e-2540.473e-022.739e-461.979e-14-6.035e-14
mm10_ENSMUSG00000023905mm10_Tnfrsf12a -7.722e-1592.965e-550.953e-050.752e-02-5.202e-08
mm10_ENSMUSG00000021559mm10_Dapk1 -7.763e-750.574e-023.123e-371.285e-04-3.919e-04
mm10_ENSMUSG00000028010mm10_Gar1 -7.802e-1013.073e-490.961e-040.532e-01-4.533e-05
mm10_ENSMUSG00000033307mm10_Mif -7.808e-1183.521e-520.372e-010.512e-01-6.126e-07
mm10_ENSMUSG00000068122mm10_Agtr2 -7.816e-75-0.256e-013.573e-391.453e-04-3.543e-03
mm10_ENSMUSG00000032826mm10_Ank2 -7.829e-1020.927e-052.275e-242.161e-13-4.562e-05
mm10_ENSMUSG00000024236mm10_Svil -7.842e-1840.993e-072.645e-451.477e-08-5.456e-10
mm10_ENSMUSG00000048706mm10_Lurap1l -7.852e-893.832e-600.167e-010.179e-01-4.589e-05
mm10_ENSMUSG00000021701mm10_Plk2 -7.863e-753.236e-350.762e-020.533e-01-4.595e-04
mm10_ENSMUSG00000028517mm10_Plpp3 -7.873e-2890.622e-032.751e-491.797e-12-6.969e-18
mm10_ENSMUSG00000022548mm10_Apod -7.891e-820.744e-032.825e-331.573e-06-4.043e-04
mm10_ENSMUSG00000027751mm10_Supt20 -7.921e-1251.284e-102.038e-222.032e-13-5.078e-07
mm10_ENSMUSG00000025364mm10_Pa2g4 -7.923e-1532.902e-531.081e-060.664e-02-5.888e-09
mm10_ENSMUSG00000064363mm10_mt-Nd4 -7.931e-2921.628e-171.664e-152.045e-14-8.863e-22
mm10_ENSMUSG00000019846mm10_Lama4 -7.952e-2030.585e-032.811e-491.741e-10-5.529e-11
mm10_ENSMUSG00000090386mm10_Mir99ahg -7.967e-820.708e-032.754e-301.733e-07-4.113e-04
mm10_ENSMUSG00000005360mm10_Slc1a3 -7.985e-2151.221e-102.112e-271.991e-14-7.302e-13
mm10_ENSMUSG00000023868mm10_Pde10a -7.981e-1131.402e-091.829e-142.149e-12-4.452e-05
mm10_ENSMUSG00000024190mm10_Dusp1 -7.996e-1551.021e-062.682e-381.366e-06-4.562e-07
mm10_ENSMUSG00000027996mm10_Sfrp2 -8.013e-1070.528e-023.022e-291.491e-04-6.333e-06
mm10_ENSMUSG00000029752mm10_Asns -8.021e-1023.832e-690.099e-010.296e-01-4.752e-05
mm10_ENSMUSG00000052310mm10_Slc39a1 -8.042e-671.426e-071.846e-102.132e-08-4.192e-03
mm10_ENSMUSG00000046668mm10_Cxxc5 -8.064e-890.772e-032.675e-311.773e-08-4.201e-04
mm10_ENSMUSG00000075602mm10_Ly6a -8.068e-870.323e-013.354e-351.193e-03-5.211e-04
mm10_ENSMUSG00000029675mm10_Eln -8.064e-760.031e+003.171e-341.834e-07-4.217e-04
mm10_ENSMUSG00000064370mm10_mt-Cytb -8.084e-2991.595e-161.691e-152.055e-14-8.471e-21
mm10_ENSMUSG00000064345mm10_mt-Nd2 -8.113e-2911.656e-171.625e-142.051e-13-8.742e-21
mm10_ENSMUSG00000064341mm10_mt-Nd1 -8.124e-3141.801e-211.542e-131.953e-13-8.661e-22
mm10_ENSMUSG00000029208mm10_Guf1 -8.127e-921.221e-072.163e-201.962e-10-4.279e-05
mm10_ENSMUSG00000057098mm10_Ebf1 -8.129e-2280.914e-062.411e-351.951e-13-5.156e-11
mm10_ENSMUSG00000029304mm10_Spp1 -8.176e-1654.003e-79-0.246e-010.354e-01-4.982e-07
mm10_ENSMUSG00000026475mm10_Rgs16 -8.182e-690.393e-013.056e-271.645e-05-4.321e-03
mm10_ENSMUSG00000031628mm10_Casp3 -8.191e-942.875e-410.888e-041.121e-03-4.338e-05
mm10_ENSMUSG00000028832mm10_Stmn1 -8.202e-793.202e-330.762e-020.592e-01-4.933e-04
mm10_ENSMUSG00000006378mm10_Gcat -8.206e-843.021e-370.779e-031.009e-03-4.353e-04
mm10_ENSMUSG00000031673mm10_Cdh11 -8.214e-1780.972e-062.622e-401.563e-08-6.532e-10
mm10_ENSMUSG00000024640mm10_Psat1 -8.252e-874.622e-81-0.764e-02-0.191e+00-4.401e-04
mm10_ENSMUSG00000061024mm10_Rrs1 -8.311e-973.365e-550.652e-020.423e-01-3.464e-04
mm10_ENSMUSG00000035783mm10_Acta2 -8.313e-1894.232e-91-0.628e-020.354e-01-5.782e-09
mm10_ENSMUSG00000101939mm10_Gm28438 -8.357e-2511.611e-151.629e-142.122e-14-8.342e-16
mm10_ENSMUSG00000029430mm10_Ran -8.389e-2403.102e-700.875e-050.586e-02-7.854e-15
mm10_ENSMUSG00000040204mm10_Pclaf -8.402e-317.849e-45-5.461e-06-1.982e-01-3.542e-01
mm10_ENSMUSG00000087365mm10_C430049B03Rik -8.441e-991.407e-101.952e-162.015e-11-4.594e-05
mm10_ENSMUSG00000032014mm10_Oaf -8.453e-1473.117e-560.827e-040.685e-02-4.922e-07
mm10_ENSMUSG00000011179mm10_Odc1 -8.491e-1073.199e-460.823e-030.512e-01-5.238e-06
mm10_ENSMUSG00000053398mm10_Phgdh -8.555e-1613.464e-750.562e-020.324e-01-4.442e-07
mm10_ENSMUSG00000024359mm10_Hspa9 -8.557e-1843.068e-630.851e-040.742e-02-6.511e-10
mm10_ENSMUSG00000064043mm10_Trerf1 -8.561e-1111.249e-092.125e-221.986e-12-4.717e-06
mm10_ENSMUSG00000062070mm10_Pgk1 -8.644e-1983.004e-620.721e-031.062e-04-4.783e-09
mm10_ENSMUSG00000064337mm10_mt-Rnr1 -8.667e-1931.661e-131.438e-092.281e-13-8.453e-12
mm10_ENSMUSG00000027533mm10_Fabp5 -8.678e-2023.084e-630.924e-050.578e-02-6.145e-11
mm10_ENSMUSG00000054717mm10_Hmgb2 -8.688e-1302.963e-430.764e-031.111e-03-5.833e-07
mm10_ENSMUSG00000076617mm10_Ighm -8.751e-63-0.374e-013.601e-361.571e-04-3.894e-03
mm10_ENSMUSG00000031548mm10_Sfrp1 -8.771e-1611.194e-082.724e-371.041e-03-6.513e-09
mm10_ENSMUSG00000031490mm10_Eif4ebp1 -8.846e-1573.232e-630.592e-020.821e-02-5.999e-09
mm10_ENSMUSG00000032085mm10_Tagln -8.943e-1483.617e-620.293e-010.462e-01-5.671e-07
mm10_ENSMUSG00000031403mm10_Dkc1 -9.172e-753.112e-330.867e-030.681e-01-4.329e-04
mm10_ENSMUSG00000052331mm10_Ankrd44 -9.192e-901.033e-052.202e-192.141e-11-4.332e-04
mm10_ENSMUSG00000031897mm10_Psmb10 -9.286e-903.303e-460.672e-020.592e-01-4.421e-04
mm10_ENSMUSG00000048930mm10_Tada3 -9.305e-961.561e-111.852e-141.956e-10-4.448e-05
mm10_ENSMUSG00000060510mm10_Zfp266 -9.592e-1151.231e-082.092e-212.056e-13-4.747e-06
This plot shows the Sequencing Saturation metric as a function of downsampled sequencing depth in mean reads per cell, up to the observed sequencing depth. Sequencing Saturation is a measure of the observed library complexity, and approaches 1.0 (100%) when all converted mRNA transcripts have been sequenced. The slope of the curve near the endpoint can be interpreted as an upper bound to the benefit to be gained from increasing the sequencing depth beyond this point. The dotted line is drawn at a value reasonably approximating the saturation point.

Sequencing Saturation

020k40k60k00.20.40.60.81
Mean Reads per CellSequencing Saturation
 
This plot shows the Median Genes per Cell as a function of downsampled sequencing depth in mean reads per cell, up to the observed sequencing depth. The slope of the curve near the endpoint can be interpreted as an upper bound to the benefit to be gained from increasing the sequencing depth beyond this point.

Median Genes per Cell

020k40k60k01000200030004000500060007000
Mean Reads per CellMedian Genes per Cellhg19mm10